3HHP
Malate dehydrogenase open conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 146.026, 51.972, 168.980 |
| Unit cell angles | 90.00, 102.23, 90.00 |
Refinement procedure
| Resolution | 60.970 - 1.450 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.065 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.971 | 1.490 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.056 | |
| Number of reflections | 215865 | |
| <I/σ(I)> | 1.5 | |
| Completeness [%] | 98.4 | 94.8 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 100 mM ADA, 0.2 M sodium acetate, 30% PEG MME 5000, 5% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
