3HHL
Crystal structure of methylated RPA0582 protein
Experimental procedure
| Experimental method | MOLECULAR REPLACEMENT |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-31 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9787 |
| Spacegroup name | H 3 |
| Unit cell lengths | 193.012, 193.012, 117.965 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.170 - 2.650 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dca |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.584 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.670 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.090 | 0.596 |
| Number of reflections | 47581 | |
| <I/σ(I)> | 24.26 | 2.3 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2M AMMONIUM SULPHATE, 25% PEG3350, 0.1M HEPES pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
