3HH8
Crystal Structure and metal binding properties of the lipoprotein MtsA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-06-18 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.260, 50.077, 157.617 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.410 - 1.870 |
| R-factor | 0.17338 |
| Rwork | 0.172 |
| R-free | 0.19862 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.466 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.900 | 1.960 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.065 | 0.320 |
| Number of reflections | 25230 | |
| <I/σ(I)> | 14.8 | 3.5 |
| Completeness [%] | 96.1 | 96 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 291 | 27% PEGMME 2K, 0.1M MgCl2, 10mM HEPES/KOH, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
