3HGZ

Crystal structure of human insulin-degrading enzyme in complex with amylin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	273
Detector technology	CCD
Wavelength(s)	0.97323
Spacegroup name	P 65
Unit cell lengths	262.868, 262.868, 90.854
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.690 - 2.910
R-factor	0.1782
Rwork	0.176
R-free	0.22388
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.406
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASES
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.982
High resolution limit [Å]	2.910	2.910
Rmerge	0.080	0.332
Number of reflections	79043
Completeness [%]	99.9	99.2
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K