3HGT

Structural and functional studies of the yeast class II Hda1 HDAC complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	103
Detector technology	PIXEL
Collection date	2008-09-02
Detector	PSI PILATUS 6M
Wavelength(s)	1.0007
Spacegroup name	P 1 21 1
Unit cell lengths	55.180, 116.570, 55.190
Unit cell angles	90.00, 97.42, 90.00

Refinement procedure

Resolution	19.983 - 2.200
R-factor	0.1995
Rwork	0.198
R-free	0.23670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.080
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.340
High resolution limit [Å]	2.200	2.200
Number of reflections	33474
Completeness [%]	95.4	88.7
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP