3HGS
Crystal structure of tomato OPR3 in complex with pHB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-15 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.610, 89.500, 80.680 |
| Unit cell angles | 90.00, 107.94, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.212 |
| Rwork | 0.206 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.081 | 0.389 |
| Number of reflections | 49387 | |
| <I/σ(I)> | 10.6 | 2 |
| Completeness [%] | 93.9 | 89.4 |
| Redundancy | 2.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 100 mM MES/Tris, 15% PEG8000, 50 mM ammonium sulfate, pH 7.5, vapor diffusion, sitting drop, temperature 298K |
