3HGM
Universal Stress Protein TeaD from the TRAP transporter TeaABC of Halomonas elongata
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 74 |
| Detector technology | CCD |
| Collection date | 2008-12-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9765 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.050, 74.650, 97.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.22 |
| Rwork | 0.218 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Poly alanine model of pdb entry 2Z09 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.075 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.081 | 0.734 |
| Number of reflections | 42968 | |
| <I/σ(I)> | 2.21 | |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 4.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 100mM Tris pH 7.5, 200mM (NH4)2SO4, 15% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
