3HGA
Crystal Structure of 4-Se-Uridine Derivatized RNA
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 30.255, 34.079, 28.931 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.470 - 1.300 |
| R-factor | 0.19414 |
| Rwork | 0.192 |
| R-free | 0.24301 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.952 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.047 | 0.185 |
| Number of reflections | 3773 | |
| <I/σ(I)> | 31.605 | |
| Completeness [%] | 96.6 | 100 |
| Redundancy | 6.8 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | MPD,sodium cacodylate,spermine tetra-HCl, vapor diffusion, hanging drop, temperature 298K |
