3HFX
Crystal structure of carnitine transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-06-28 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0039 |
| Spacegroup name | P 63 |
| Unit cell lengths | 134.210, 134.210, 85.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 3.150 |
| Rwork | 0.262 |
| R-free | 0.28100 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.470 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.042 | 3.320 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.070 | 0.391 |
| Total number of observations | 109568 | |
| Number of reflections | 29078 | |
| <I/σ(I)> | 17.5 | 4.4 |
| Completeness [%] | 98.2 | 98 |
| Redundancy | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1M HEPES-NAOH (PH7.5), 22-26% PEG 400, 0.1M NaCl, 18mM n-octyl-D-glucopyranoside (-OG), 5mM L-Carnitine, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 289K |
