3HFQ
Crystal structure of the lp_2219 protein from Lactobacillus plantarum. Northeast Structural Genomics Consortium Target LpR118.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97860, 0.97917, 0.91837 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.543, 54.029, 79.584 |
| Unit cell angles | 90.00, 104.18, 90.00 |
Refinement procedure
| Resolution | 31.447 - 1.963 |
| R-factor | 0.17 |
| Rwork | 0.169 |
| R-free | 0.17800 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.475 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXDE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 2.040 |
| High resolution limit [Å] | 1.963 | 1.970 |
| Rmerge | 0.041 | 0.074 |
| Number of reflections | 87372 | |
| <I/σ(I)> | 22.2 | 13.6 |
| Completeness [%] | 96.7 | 90.4 |
| Redundancy | 1.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch under oil | 291 | 20% PEG 3350, 0.2M ammonium phosphate, Microbatch under oil, temperature 291K |
