3HEJ
Crystal structure of Staphylococcal nuclease variant Delta+PHS T62R at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.598, 74.420, 67.975 |
| Unit cell angles | 90.00, 90.22, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.239 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.3) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.040 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.056 | |
| Number of reflections | 62385 | |
| <I/σ(I)> | 22.9 | |
| Completeness [%] | 99.3 | 99.4 |
| Redundancy | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 24% MPD, 25 mM Potassium Phosphate, Calcium Chloride, 5% Glycerol, pdTp, pH 8.0, vapor diffusion, hanging drop, temperature 277K |
