3HEF
Crystal structure of the bacteriophage Sf6 terminase small subunit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-26 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.02462 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 88.858, 88.858, 72.631 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.650 |
Rwork | 0.224 |
R-free | 0.25100 |
Structure solution method | MAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | RESOLVE (2.13) |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.053 | 0.521 |
Number of reflections | 35647 | |
<I/σ(I)> | 107.121 | |
Completeness [%] | 99.7 | 100 |
Redundancy | 28.4 | 28.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.2M NaH2PO4/0.8M K2HPO4, 0.2M Li2SO4, 5% glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.2M NaH2PO4/0.8M K2HPO4, 0.2M Li2SO4, 5% glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |