3HE8
Structural study of Clostridium thermocellum Ribose-5-Phosphate Isomerase B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6C1 |
Synchrotron site | PAL/PLS |
Beamline | 6C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-18 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9999 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 69.515, 69.515, 154.434 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.810 - 1.900 |
R-factor | 0.18924 |
Rwork | 0.188 |
R-free | 0.22011 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vvr |
RMSD bond length | 0.015 |
RMSD bond angle | 1.332 |
Phasing software | swiss-model |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.035 | 0.051 |
Number of reflections | 32228 | |
<I/σ(I)> | 63.1 | 63.1 |
Completeness [%] | 92.2 | 92.2 |
Redundancy | 21.1 | 21.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 283 | 0.05M Tris, pH7.0, 10% PEG8000, 0.15M Magnesium chloride, 0.2M Potassium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 283K |