3HE1
Secreted protein Hcp3 from Pseudomonas aeruginosa.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-17 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 3 1 2 |
| Unit cell lengths | 141.219, 141.219, 105.052 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.800 - 2.098 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.25400 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.189 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.800 | 2.140 |
| High resolution limit [Å] | 2.098 | 2.100 |
| Rmerge | 0.120 | 0.647 |
| Number of reflections | 69851 | |
| <I/σ(I)> | 28.392 | 4.09 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.4 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.1 M Bis-Tris buffer, 3.2 M sodium chloride, chymotrypsin partial digestion in crystallization droplet, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
