3HE0
The Structure of a Putative Transcriptional Regulator TetR Family Protein from Vibrio parahaemolyticus.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97942, 0.97931 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.272, 95.548, 190.541 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.670 - 2.200 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.22700 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.436 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.089 | 0.580 |
| Number of reflections | 34848 | |
| <I/σ(I)> | 33.882 | |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 9.5 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.8M ammonium sulfate,0.1M tri-sodium citrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
