3HDV
Crystal structure of response regulator receiver protein from Pseudomonas putida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-15 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 110.769, 140.436, 48.571 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.500 - 2.090 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.24500 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.875 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.500 | 2.180 |
| High resolution limit [Å] | 2.090 | 2.100 |
| Rmerge | 0.096 | 0.520 |
| Number of reflections | 45411 | |
| <I/σ(I)> | 10.3 | 5 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 14.2 | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 298 | 0.1M HEPES pH7.5, 0.8M Potassium Sodium Tartarate tetrahydrate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
