3HDG
Crystal structure of the N-terminal domain of an uncharacterized protein (WS1339) from Wolinella succinogenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9790 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.331, 77.059, 75.087 |
Unit cell angles | 90.00, 102.67, 90.00 |
Refinement procedure
Resolution | 38.530 - 2.270 |
Rwork | 0.223 |
R-free | 0.27000 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELX (& SHARP) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.220 | 2.350 |
High resolution limit [Å] | 2.270 | 2.270 |
Rmerge | 0.107 | 0.560 |
Number of reflections | 24794 | |
<I/σ(I)> | 10.6 | |
Completeness [%] | 99.5 | 95.6 |
Redundancy | 7.2 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 30% PEG MME 550, 50mM Magnesium Chloride, 100mM HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |