3HDA
PrtC methionine mutants: M226A_DESY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.812 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 101.858, 101.858, 122.521 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.508 - 2.131 |
| R-factor | 0.183 |
| Rwork | 0.182 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1go8 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.882 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | PHENIX (1.4_54) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.170 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.083 | 0.396 |
| Number of reflections | 41603 | |
| <I/σ(I)> | 19.709 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2 M NaCl, 0.1 M phosphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
