3HD0

Crystal structure of Tm1865, an Endonuclease V from Thermotoga Maritima

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-27
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97876, 0.97860, 0.97243
Spacegroup name	P 21 21 21
Unit cell lengths	69.571, 71.966, 120.266
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.700
R-factor	0.16742
Rwork	0.163
R-free	0.25518
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.635
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.13)
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.131	0.453
Number of reflections	17147
<I/σ(I)>	15.333	3.27
Completeness [%]	99.8	99.8
Redundancy	5.3	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	Crystallization drop was 1:1 mixture of protein dissolved in 20 mM Tris pH 8.5, 100 mM NaCl and 24 w/v% PEG 1500, 20% glycerol. The crystallization reservoir was 1.5 M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 298K