3HBM
Crystal Structure of PseG from Campylobacter jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 41 |
| Unit cell lengths | 93.647, 93.647, 42.702 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.110 - 1.800 |
| R-factor | 0.1981 |
| Rwork | 0.196 |
| R-free | 0.23458 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.261 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.110 | 1.870 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 34208 | |
| <I/σ(I)> | 22.6 | 8.4 |
| Completeness [%] | 98.8 | 97.7 |
| Redundancy | 7.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Bis-Tris, 0.2M Ammonium sulfate, 23% (v/v) PEG monomethyl ether 550, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
