3HB4
17beta-hydroxysteroid dehydrogenase type1 complexed with E2B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 277 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.080, 43.700, 60.510 |
| Unit cell angles | 90.00, 100.74, 90.00 |
Refinement procedure
| Resolution | 18.980 - 2.210 |
| R-factor | 0.19409 |
| Rwork | 0.191 |
| R-free | 0.25739 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.235 |
| Data reduction software | XDS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.980 | 2.263 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Number of reflections | 15658 | |
| <I/σ(I)> | 0.045 | |
| Completeness [%] | 97.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 300 | pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
