3HAY
Crystal structure of a substrate-bound full H/ACA RNP from Pyrococcus furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Collection date | 2006-11-15 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 189.518, 189.518, 279.045 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 4.990 |
| R-factor | 0.32494 |
| Rwork | 0.323 |
| R-free | 0.36657 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hax |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.971 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 5.180 |
| High resolution limit [Å] | 4.990 | 5.000 |
| Rmerge | 0.082 | 0.481 |
| Number of reflections | 13408 | |
| <I/σ(I)> | 34.4 | 4.7 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 293 | 1.5M lithium sulfate, 50mM sodium acetate (pH 4.9), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
