3H9Z
Crystal structure of the IgE-Fc3-4 domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 1999-08-07 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.0001 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.660, 99.352, 77.639 |
| Unit cell angles | 90.00, 97.40, 90.00 |
Refinement procedure
| Resolution | 28.710 - 2.450 |
| R-factor | 0.23182 |
| Rwork | 0.229 |
| R-free | 0.27748 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fp5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.448 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Number of reflections | 36017 | |
| <I/σ(I)> | 18.425 | |
| Completeness [%] | 98.2 | 82.3 |
| Redundancy | 3.63 | 2.28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 293 | 1 microliter of protein at 10 mg/mL in 20 mM sodium chloride was added to 1 microliter of well solution (100 mM ammonium acetate, 100 mM sodium acetate pH 4.6, 30% (w/v) PEG 4000) and mixed by pipetting., VAPOR DIFFUSION, HANGING DROP, temperature 293K |
