3H9X
Crystal Structure of the PSPTO_3016 protein from Pseudomonas syringae, Northeast Structural Genomics Consortium Target PsR293
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97896 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.302, 48.546, 68.047 |
| Unit cell angles | 87.07, 92.40, 93.57 |
Refinement procedure
| Resolution | 19.940 - 2.510 |
| R-factor | 0.212 |
| Rwork | 0.210 |
| R-free | 0.24500 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2 & XtalView) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.071 | 0.255 |
| Number of reflections | 37487 | |
| <I/σ(I)> | 12.18 | 2.37 |
| Completeness [%] | 96.9 | 93.5 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 277 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution: 100mM NaCitrate (pH 4.2), 20% PEG8k, and 60mM NaNO3. , VAPOR DIFFUSION, HANGING DROP, temperature 277K |
