3H9V
Crystal structure of the ATP-gated P2X4 ion channel in the closed, apo state at 3.1 Angstroms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-26 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 100.836, 100.836, 431.316 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.109 - 3.100 |
| R-factor | 0.2494 |
| Rwork | 0.248 |
| R-free | 0.27820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.483 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.200 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Rmerge | 0.080 | 0.484 |
| Number of reflections | 14414 | |
| <I/σ(I)> | 18.2 | 1.8 |
| Completeness [%] | 90.1 | 69.9 |
| Redundancy | 3.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.4 | 277 | 20% PEG 2,000, 300mM Mg(NO3)2, and 100mM Tris pH 8.4, and 1mM GdCl3, vapor diffusion, temperature 277K |
