3H9R
Crystal structure of the kinase domain of type I activin receptor (ACVR1) in complex with FKBP12 and dorsomorphin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9050 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.349, 62.345, 171.597 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.150 - 2.350 |
| R-factor | 0.19022 |
| Rwork | 0.187 |
| R-free | 0.25594 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1A7X CHAIN A 1B6C CHAIN B |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.469 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.150 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.160 | 0.684 |
| Number of reflections | 20161 | |
| <I/σ(I)> | 7.4 | 2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277.15 | 30% PEG 3350; 0.25M Ammonium sulphate; 0.1M Bis-Tris, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
