3H99
Structure of a mutant methionyl-tRNA synthetase with modified specificity complexed with methionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.172, 45.293, 85.967 |
| Unit cell angles | 90.00, 107.25, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.400 |
| R-factor | 0.168 |
| Rwork | 0.168 |
| R-free | 0.18000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h97 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.222 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.025 | 0.075 |
| Number of reflections | 111291 | 20621 |
| <I/σ(I)> | 28.8 | 11.7 |
| Completeness [%] | 98.0 | 97.8 |
| Redundancy | 2.8 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 279 | Ammonium citrate, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 279K |
