3H93
Crystal Structure of Pseudomonas aeruginosa DsbA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-08 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95364 |
| Spacegroup name | P 41 |
| Unit cell lengths | 41.155, 41.155, 98.318 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.106 - 1.501 |
| R-factor | 0.112 |
| Rwork | 0.110 |
| R-free | 0.15200 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.969 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | RESOLVE (2.13) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.076 | 0.361 |
| Number of reflections | 51579 | |
| <I/σ(I)> | 46.483 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 28 | 17.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 24% w/v PEG 1500, 22% v/v Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
