3H8W

Structure of D132N T4 RNase H in the presence of divalent magnesium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	53.629, 74.572, 88.242
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.340 - 2.800
R-factor	0.231
Rwork	0.229
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1TFR native T4 RNase H
RMSD bond length	0.002
RMSD bond angle	0.498
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Phasing software	AMoRE
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.340	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.041	0.177
Total number of observations		4280
Number of reflections	8890
<I/σ(I)>	15.3	4.3
Completeness [%]	97.4	94.5
Redundancy	3.6	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	100 mM Na PIPES, 200 mM MgCl2, 14% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K