3H8N
Crystal Structure Analysis of KIR2DS4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-07 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.232, 62.851, 65.934 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.450 - 2.500 |
| R-factor | 0.241 |
| Rwork | 0.238 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1im9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.358 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.450 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 7795 | |
| <I/σ(I)> | 64.4 | 9.7 |
| Completeness [%] | 99.8 | 97 |
| Redundancy | 13.7 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 283 | 0.22 M Mg-Formate, 100 mM BIS-TRIS, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
