3H8K
Crystal structure of Ube2g2 complxed with the G2BR domain of gp78 at 1.8-A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-21 |
| Detector | MAR300 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.920, 60.150, 61.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.048 - 1.800 |
| R-factor | 0.198 |
| Rwork | 0.193 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cyx |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.921 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.1) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.078 | 0.437 |
| Number of reflections | 16657 | |
| <I/σ(I)> | 22.381 | 2.74 |
| Completeness [%] | 95.4 | 74.2 |
| Redundancy | 6.4 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | PEG 3350, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
