3H8D
Crystal structure of Myosin VI in complex with Dab2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-10-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.177, 69.952, 78.537 |
| Unit cell angles | 90.00, 98.02, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.25100 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.092 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 77.850 | 34.990 | 2.320 |
| High resolution limit [Å] | 2.200 | 6.960 | 2.200 |
| Rmerge | 0.084 | 0.026 | 0.548 |
| Total number of observations | 135314 | 4272 | 18948 |
| Number of reflections | 36631 | ||
| <I/σ(I)> | 8.893 | 19.8 | 1.4 |
| Completeness [%] | 97.0 | 98 | 95.3 |
| Redundancy | 3.7 | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 20% PEG3350, 0.2M potassium thiocyanate for Myosin VI and 20% PEG3350, 0.2M sodium iodide for Dab2, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
