3H8A
Crystal structure of E. coli enolase bound to its cognate RNase E recognition domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 103.883, 110.207, 160.267 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.890 - 1.900 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e9i |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.570 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.890 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 136948 | |
| Completeness [%] | 99.3 | 95.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 291 | 0.1 M HEPES, 1.6 M NH4SO4, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
