3H7I
Structure of the metal-free D132N T4 RNase H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.011, 85.858, 57.260 |
| Unit cell angles | 90.00, 92.00, 90.00 |
Refinement procedure
| Resolution | 35.989 - 1.502 |
| R-factor | 0.1809 |
| Rwork | 0.180 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1TFR native T4 RNase H |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.320 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.048 | 0.089 |
| Number of reflections | 52759 | |
| <I/σ(I)> | 27.9 | 10 |
| Redundancy | 5.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 200 mM ammonium acetate, 100 mM sodium cacodylate, 12% PEG 8000, 5% 2-methyl-2,4-pentanediol (MPD), pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
