3H5Z
Crystal Structure of Leishmania major N-myristoyltransferase with bound myristoyl-CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.816, 91.549, 53.625 |
| Unit cell angles | 90.00, 111.90, 90.00 |
Refinement procedure
| Resolution | 39.920 - 1.490 |
| R-factor | 0.1887 |
| Rwork | 0.187 |
| R-free | 0.21958 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p6e |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.216 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Number of reflections | 68305 | |
| <I/σ(I)> | 10.5 | 1 |
| Completeness [%] | 98.7 | 93.7 |
| Redundancy | 3.3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 25.9% PEG1500, 0.2M NaCl, 0.1M NaCacodylate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
