3H51

Crystal structure of Putative calcium/calmodulin dependent protein kinase II association domain (NP_636218.1) from XANTHOMONAS CAMPESTRIS at 1.70 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-18
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97901
Spacegroup name	P 1 21 1
Unit cell lengths	38.832, 80.550, 51.431
Unit cell angles	90.00, 95.59, 90.00

Refinement procedure

Resolution	29.488 - 1.700
R-factor	0.18
Rwork	0.179
R-free	0.21500
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.669
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.5)
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.488	29.490	1.740
High resolution limit [Å]	1.700	7.600	1.700
Rmerge	0.074	0.047	0.665
Total number of observations		1157	7906
Number of reflections	33865
<I/σ(I)>	6.464	13.3	1.2
Completeness [%]	97.9	94.4	97.3
Redundancy	3.2	3.1	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	20.0000% PEG-1000, 0.1M TRIS pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K