3H4P
Proteasome 20S core particle from Methanocaldococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 206.720, 219.540, 149.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.975 - 4.100 |
| R-factor | 0.2573 |
| Rwork | 0.254 |
| R-free | 0.32450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.250 |
| High resolution limit [Å] | 4.100 | 4.100 |
| Number of reflections | 54076 | |
| <I/σ(I)> | 9.5 | 3.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 295 | 0.1 M Tris, pH 6.8, 0.2 M KCl and 33-36% MPD (v/v), VAPOR DIFFUSION, HANGING DROP, temperature 295K |
