3H4L

Crystal Structure of N terminal domain of a DNA repair protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-06-10
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	75.592, 103.288, 117.167
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.870 - 2.500
R-factor	0.235
Rwork	0.235
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hs7
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MLPHARE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	30119
<I/σ(I)>	17	2.2
Completeness [%]	97.7	99.4
Redundancy	3.4	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch	8.5	277	.1M Tris, 10% PEG 4K, 0.2M sodium acetate, pH 8.5, Batch, temperature 277K