3H4H
Met94Thr/Phe312Cys variant of nitrite reductase from Alcaligenes faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Collection date | 2004-06-19 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.301, 102.077, 146.336 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.18281 |
| Rwork | 0.181 |
| R-free | 0.22594 |
| Structure solution method | Refinement of wild-type structure |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.386 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 84.520 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 103196 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
