3H47

X-ray Structure of Hexameric HIV-1 CA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Collection date	2008-07-18
Wavelength(s)	1.000
Spacegroup name	P 6
Unit cell lengths	90.995, 90.995, 56.853
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.740 - 1.900
R-factor	0.2342
Rwork	0.232
R-free	0.26950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 2PXR - with residues 1-14 and 87-100 deleted PDB 2BUO - with residues 175-190 deleted
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900
Number of reflections	21352
<I/σ(I)>	21.1
Completeness [%]	99.9
Redundancy	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	10-12% PEG 8,000, 100 mM sodium malonate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K