3H45
Glycerol Kinase H232E with Ethylene Glycol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 93 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 2007-06-08 |
| Detector | RIGAKU AFC11-KAPPA |
| Wavelength(s) | 1.514 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.606, 105.194, 114.305 |
| Unit cell angles | 90.00, 114.55, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.650 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xup |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.361 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 73411 | |
| <I/σ(I)> | 6.5 | 1.7 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | KH2PO4, PEG 8000, VAPOR DIFFUSION, temperature 298K |
