3H3V
Yeast RNAP II containing poly(A)-signal sequence in the active site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-18 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.918905 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 222.470, 391.620, 284.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 4.000 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y1w |
RMSD bond length | 0.008 |
RMSD bond angle | 1.480 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.250 |
High resolution limit [Å] | 4.000 | 4.000 |
Number of reflections | 202368 | |
<I/σ(I)> | 9.6 | 2.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.3 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 295 | 200 mM ammonium acetate, 50mM HEPES pH 7.0, 4% polyethylene glycol 6000, 5mM TCEP (tris (2-carboxyethyl) phosphine hydrochloride), VAPOR DIFFUSION, HANGING DROP, temperature 295.0 K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | ammonium acetate | ||
2 | 1 | 2 | ammonium acetate | ||
3 | 1 | 1 | HEPES | ||
4 | 1 | 2 | HEPES | ||
5 | 1 | 1 | polyethylene glycol 6000 | ||
6 | 1 | 2 | polyethylene glycol 6000 | ||
7 | 1 | 1 | TCEP (tris (2-carboxyethyl) phosphine hydrochloride) | ||
8 | 1 | 2 | TCEP (tris (2-carboxyethyl) phosphine hydrochloride) |