3H3U
Crystal structure of CRP (cAMP receptor Protein) from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 94 |
| Detector technology | CCD |
| Collection date | 2005-10-24 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.064, 84.618, 101.215 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.319 - 2.900 |
| R-factor | 0.2265 |
| Rwork | 0.223 |
| R-free | 0.29660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d0s |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.631 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.860 | 3.200 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.080 | 0.273 |
| Number of reflections | 10723 | |
| <I/σ(I)> | 16.2 | 5.1 |
| Completeness [%] | 99.9 | |
| Redundancy | 15.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 0.2M Lithium Sulphate, 15% Ethanol, 0.1M Sodium Citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
