3H3P
Crystal structure of HIV epitope-scaffold 4E10 Fv complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 107 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-08-13 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 92.400, 92.400, 272.790 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.080 - 2.700 |
| R-factor | 0.23171 |
| Rwork | 0.229 |
| R-free | 0.28697 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | computationally-derived model of the Fv based on 1TZG |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.109 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.020 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.104 | 0.347 |
| Number of reflections | 19557 | |
| <I/σ(I)> | 7.5 | 2.9 |
| Completeness [%] | 98.6 | 99.6 |
| Redundancy | 4.56 | 4.29 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 0.2 M calcium acetate, 8% PEG 8000, 0.1 M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
