3H3D
Drosophila Pumilio RNA binding domain (Puf domain)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2000-06-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1 |
Spacegroup name | P 63 |
Unit cell lengths | 94.580, 94.580, 229.270 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.780 - 2.300 |
R-factor | 0.254 |
Rwork | 0.252 |
R-free | 0.29800 |
Structure solution method | MAD |
Starting model (for MR) | none |
RMSD bond length | 0.033 |
RMSD bond angle | 2.610 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.089 | 0.311 |
Number of reflections | 49178 | |
<I/σ(I)> | 20.7 | 2.4 |
Completeness [%] | 95.8 | 73.2 |
Redundancy | 8.9 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | 2.1M Ammonium sulfate, 100mM HEPES, 4% DMSO, 2mM DTT, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
2 |