3H36
Structure of an uncharacterized domain in polyribonucleotide nucleotidyltransferase from Streptococcus mutans UA159
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97945,0.97931 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 59.312, 59.312, 93.090 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.990 - 1.800 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.23600 |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.538 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.082 | 0.671 |
| Number of reflections | 11267 | |
| <I/σ(I)> | 65.5 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 22.7 | 22.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | 0.1M Na Cacodylate pH6.5,0.2M Ca Acetate, 40% PEG 3000, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
