3H2T
Crystal structure of gene product 6, baseplate protein of bacteriophage T4
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97948 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.774, 94.579, 136.221 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.856 - 3.200 |
| R-factor | 0.2344 |
| Rwork | 0.230 |
| R-free | 0.32160 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.215 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.096 | 0.476 |
| Number of reflections | 16878 | |
| <I/σ(I)> | 26.9 | 3.9 |
| Completeness [%] | 99.6 | 97 |
| Redundancy | 7.2 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | 1.2 M Ammonium Sulfate, buffered with HEPES, pH 7.0, VAPOR DIFFUSION, temperature 298K |
| 1 | VAPOR DIFFUSION | 7 | 298 | 1.2 M Ammonium Sulfate, buffered with HEPES, pH 7.0, VAPOR DIFFUSION, temperature 298K |
