3H2B
Crystal structure of the SAM-dependent methyltransferase cg3271 from Corynebacterium glutamicum in complex with S-adenosyl-L-homocysteine and pyrophosphate. Northeast Structural Genomics Consortium Target CgR113A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97869 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.827, 72.424, 74.826 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.970 - 2.000 |
R-factor | 0.174 |
Rwork | 0.173 |
R-free | 0.22600 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.121 | 0.525 |
Number of reflections | 50463 | |
<I/σ(I)> | 14.14 | 2.62 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.4 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH UNDER OIL | 6.5 | 277 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl pH 7.5. Reservoir solution: 100mM Sodium cacodylate pH 6.5, 25% PEG 8000, 200mM Ammonium sulfate, MICROBATCH UNDER OIL, temperature 277K |