3H1Z
Molecular basis for the association of PIPKIgamma -p90 with the clathrin adaptor AP-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-12 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.561, 83.469, 91.603 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.250 - 1.830 |
R-factor | 0.196 |
Rwork | 0.193 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g30 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.618 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.900 |
High resolution limit [Å] | 1.830 | 3.940 | 1.830 |
Rmerge | 0.060 | 0.028 | 0.461 |
Number of reflections | 24788 | ||
<I/σ(I)> | 20.383 | ||
Completeness [%] | 94.6 | 97.1 | 88.1 |
Redundancy | 4.5 | 4.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 18% PEG8000, 100mM HEPES, pH7.5, 4mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |