3H1F
Crystal structure of CheY mutant D53A of Helicobacter pylori
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-11-15 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 70.335, 38.081, 38.639 |
Unit cell angles | 90.00, 107.35, 90.00 |
Refinement procedure
Resolution | 15.530 - 2.200 |
R-factor | 0.1824 |
Rwork | 0.180 |
R-free | 0.23256 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gwg |
RMSD bond length | 0.019 |
RMSD bond angle | 1.828 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | PHASER |
Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.880 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.082 | 0.240 |
Number of reflections | 4993 | |
<I/σ(I)> | 10.6 | 4.5 |
Completeness [%] | 98.5 | 97 |
Redundancy | 3.78 | 3.82 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 0.1M sodium acetate, 35% MPEG2000, 0.05M ammonium sulfate, 1mM Magnesium chloride, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |